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KMID : 0613820020120060812
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Journal of Life Science
2002 Volume.12 No. 6 p.812 ~ p.820
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Isolation of an acid-tolerant Leuconostoc mesenteroides LM3 from Kimchi
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Sa Geum-Hee
Paik Sang-Kyoo
Yun Hae-Sun
Kang Kyung-Hee
Jung Jin-Kook
Kim Il-Sup
Moon Hye-Yon
Kim Sa-Yeul
Yu Choon-Bal
Jin Ingn-Yol
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Abstract
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In order to understand stress response of Leuconostoc mesenteroides against lactic acid, a new Leuconostoc sp. which has acid tolerance was isolated from various Kimchi samples. And it identified as Leuconostoc mesenteroides LM3 by comparing its fatty acid composition with reference strain. Its growth pattern was investigated by adding a given concentration of lactic acid at the lag phase to the stationary phase. In the DeMan, Rogosa, Sharpe (MRS) media containing over 0.4% (final v/v) lactic acid, this strain growed slowly. After exposure of the stationary phase cells to 4% of lactic acid for 60 min, this strain could survive, whereas a reference strain, Leuconostoc mesenteroides KCTC3505, showed no survival. And changes of trehalose concentration, the activity of trehalase and ATPase in the growth of Leuconostoc mesenteroides LM3 after addition of 0.6% (final v/v) lactic acid were investigated : After exposure to lactic acid, trehalose concentration in this strain was increased in comparison with no treatment, but its trehalase activity was not changed. And its ATPase activity was constant, and intracellular pH was almost constant. This result meant Leuconostoc mesenteroides LM3 should have a tolerance against lactic acid. It remains to further study the mechanism of this acid tolerance.
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KEYWORD
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Leuconostoc, acid tolerance, lactic acid, Kimchi
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